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KMID : 1007520150240051805
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Food Science and Biotechnology
2015 Volume.24 No. 5 p.1805 ~ p.1810
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Characterization of the Starch Degradation Activity from Newly Isolated Rhizopus oryzae WCS-1 and Mixed Cultures with Saccharomyces cerevisiae for Efficient Ethanol Production from Starch
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Jang Seung-Won
Kim Jin-Seong
Park Jae-Bum
Jung Jong-Hyun
Park Cheon-Seok
Shin Won-Cheol
Ha Suk-Jin
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Abstract
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A starch degrading fungi was isolated from nuruk, a Korean traditional starter culture made with wheat flour. This newly isolated fungi was identified as Rhizopus oryzae WCS-1 via internal transcribed spacer region sequencing. Enzymatic assays for starch degradation activities indicated an optimum temperature and pH of 55°C and pH 4.0, respectively, using partially purified enzymes from R. oryzae WCS-1. Mixed cultures with S. cerevisiae CEN-PK 2-1D, L2612, BY4742, and D452-2 were performed. S. cerevisiae CEN-PK 2-1D has a maltose utilization pathway and showed the highest ethanol production rate with low maltose concentrations. Addition of 5 mM NaCl to mixed cultures of R. oryzae WCS-1 and S. cerevisiae CEN-PK 2-1D resulted in 21 and 33% improvements in the starch consumption rate and the ethanol production rate, respectively, because Na+ stimulated the starch degradation activity of partially purified enzymes from R. oryzae WCS-1.
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KEYWORD
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Rhizopus oryzae, starch, glucoamylase, mixed culture
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